The crash happened Wednesday along southbound Interstate 65 in Jackson County near the 54 mile marker north of Seymour.
Indiana State Police said in statement that a semi-truck slowed in the left lane and a pickup driven by 67-year-old John W. Mumma of Philo, Illinois, slowed behind it. A recreational vehicle driven by 57-year-old Glenn E. Cardelli of Minocqua, Wisconsin, that also was carrying his wife, 57-year-old wife Kathryn L. Cardelli, slowed behind them.
A semi-truck failed to slow and hit the recreational vehicle, causing a chain-reaction crash. Police say Mumma and the Cardellis died in the crash.
No one else was injured. The crash is under investigation.